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Updated: Mar 30, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Efficient Generation of Mice with Consistent Transgene Expression by FEEST.

Lei Gao^1,2, Yonghua Jiang², Libing Mu³

¹College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Scientific Reports

|November 18, 2015

Summary

A new method, functionally enriched ES cell transgenics (FEEST), enables efficient generation of transgenic mice with guaranteed gene expression. This technique ensures single-copy transgene integration, advancing biomedical research and disease modeling.

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Last Updated: Mar 30, 2026

Generation of Genetically Modified Mice through the Microinjection of Oocytes

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Published on: June 15, 2017

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Published on: April 2, 2020

Area of Science:

Biotechnology
Genetics
Animal Models

Background:

Transgenic mouse models are crucial for biomedical research.
Current methods face limitations due to unpredictable transgene expression and integration.

Purpose of the Study:

To introduce a novel, highly efficient technique for generating transgenic mice.
To ensure single-copy transgene integration and guaranteed gene expression.

Main Methods:

Functionally enriched ES cell transgenics (FEEST) utilizes an inducible Cre gene in ES cells.
Selection of transgenic clones is achieved using hygromycin prior to Cre-mediated recombination.
Gene-of-interest (GOI) expression is confirmed via GFP assay after recombination.

Main Results:

FEEST demonstrated efficient generation of transgenic mouse clones.
A Cre-activatable tTA (cl-tTA6) mouse model was successfully produced.
The cl-tTA6 model faithfully recapitulated tumor development and was adaptable for other genes like EGFR L858R.

Conclusions:

FEEST offers a robust and efficient method for creating transgenic mice with predictable gene expression.
This technique facilitates the generation of precise disease models for various genes.
FEEST holds potential for genome-wide scale generation of transgenic mouse models.