Related Experiment Video

Updated: Mar 30, 2026

Experimental Metastasis Assay
08:28

Experimental Metastasis Assay

Published on: August 24, 2010

21.1K

New function of TSGA10 gene in angiogenesis and tumor metastasis: a response to a challengeable paradox

Kamran Mansouri1, Ali Mostafie2, Davood Rezazadeh1

  • 1Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran and Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Human Molecular Genetics
|November 18, 2015
PubMed

Insights

Testis-specific gene antigen (TSGA10) acts as a tumor suppressor by inhibiting angiogenesis and invasion. Overexpression of TSGA10 disrupts the hypoxia-inducible factor (HIF-1α) pathway, offering therapeutic potential for cancer management.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Testis-specific gene antigen (TSGA10) has a debated role, with some studies classifying it as a cancer testis antigen (CTA) and others as a tumor suppressor.
  • The interaction between TSGA10 and hypoxia-inducible factor 1-alpha (HIF-1α) is not fully understood, leading to questions about TSGA10's precise function in tumor angiogenesis and invasion.

Purpose of the Study:

  • To investigate the function of the TSGA10 gene.
  • To evaluate the effects of TSGA10 on tumor angiogenesis and invasion.

Main Methods:

  • TSGA10 vector was stably transfected into HeLa cells, followed by clonal selection.
  • Gene expression and protein levels were assessed using real-time PCR, western blot, zymography, and ELISA under normoxia and hypoxia.
  • Invasion, migration, and angiogenesis were evaluated, alongside 3D protein modeling and molecular docking of TSGA10 with HIF-1α domains.

Main Results:

  • Increased TSGA10 expression correlated with reduced HIF-1α transcriptional activity.
  • TSGA10 overexpression inhibited angiogenesis and invasion of HeLa cells in both normoxic and hypoxic conditions.
  • Molecular docking revealed a stronger binding affinity of TSGA10 to the TAD-C (CBP) domain of HIF-1α compared to the PAS-B domain.

Conclusions:

  • Overexpression of TSGA10 disrupts the HIF-1α signaling axis.
  • TSGA10 exhibits potent inhibitory effects on tumor angiogenesis and metastasis.
  • TSGA10 holds promise as a therapeutic candidate, prognostic factor, and cancer management tool.

More Related Videos

Spheroid Assay to Measure TGF-β-induced Invasion09:18

Spheroid Assay to Measure TGF-β-induced Invasion

Published on: November 16, 2011

22.6K
Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

2.4K

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
4.0K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.9K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
7.6K
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
8.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
10.0K