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CMTM8 is Frequently Downregulated in Multiple Solid Tumors

Wenjuan Zhang1, Hui Qi, Xiaoning Mo

  • 1*Department of Immunology, School of Basic Medical Sciences, Key Laboratory of Immunology, Ministry of Health, Peking University Health Science Center †National Institutes for Food and Drug Control ‡Peking University People's Hospital, Beijing, P.R. China.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|November 18, 2015
PubMed

Insights

CMTM8 is widely expressed in normal human tissues but often lost in solid tumors like liver and lung cancer. This study reveals CMTM8

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Previous studies suggest CMTM8 influences cell growth, apoptosis, and epithelial-to-mesenchymal transition (EMT).
  • The expression pattern of CMTM8 in normal versus tumorous human tissues remains largely uncharacterized.
  • Understanding CMTM8 expression is crucial for its potential role in tumorigenesis and metastasis.

Purpose of the Study:

  • To extensively analyze the expression of CMTM8 in various normal human tissues.
  • To investigate the expression levels of CMTM8 in multiple types of human solid tumors compared to adjacent normal tissues.
  • To determine the correlation between CMTM8 expression and tumorigenesis.

Main Methods:

  • Real-time quantitative polymerase chain reaction (RT-qPCR) for mRNA expression analysis in normal tissues.
  • High-throughput immunohistochemical (IHC) staining of tissue microarrays (TMAs) to assess protein expression in tumor and normal samples.
  • Statistical analysis (Chi-squared tests) to evaluate the association between CMTM8 expression and tumorigenesis.

Main Results:

  • CMTM8 is broadly expressed across many normal human tissues.
  • CMTM8 expression is frequently downregulated or absent in solid tumors, including liver, lung, colon, rectum, esophagus, and stomach.
  • A significant negative correlation was observed between CMTM8 expression and the presence of tumors in multiple cancer types (P<0.0001 to P<0.01).
  • RT-qPCR confirmed reduced CMTM8 mRNA levels in esophageal carcinomas compared to adjacent normal tissues (P<0.01).

Conclusions:

  • This is the first comprehensive study detailing CMTM8 expression in both normal and cancerous human tissues.
  • The findings strongly support a potential role for CMTM8 as a novel tumor suppressor gene.
  • Downregulation of CMTM8 may be a significant event in the development of various solid tumors.

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