Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

3.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
3.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

5.1K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

6.2K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.2K
Abnormal Proliferation02:23

Abnormal Proliferation

5.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
MicroRNAs01:22

MicroRNAs

24.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.6K
MicroRNAs01:22

MicroRNAs

4.3K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Supramolecular Reactivation of Quenched Silicon Naphthalocyanine for NIR-II Fluorescence-Guided Type I/II Photodynamic Monotherapy.

ACS applied materials & interfaces·2026
Same author

Tuning Photophysical Conflict Into Synergy: Stereoelectronic Planarity Disruption Unlocks Concurrent Aggregation-Enhanced ROS and NIR-II Fluorescence.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

The "Iron Gate" Outcompetes the "Enzymic Latch" as the Dominant Soil Organic Carbon Stabilization Mechanism in Permafrost Peatlands of the Great Hing'an Mountains.

Biology·2025
Same author

Response of Soil Microbial Communities Between Different Vegetation Types in the Greater and Lesser Khingan Mountains Ecotone in Northeast China.

Microorganisms·2025
Same author

Achieving Ultrabright NIR-II Nanofluorophore for In Vivo Imaging by Inhibiting H-Aggregates Formation.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024
Same author

Distribution and ecological risks of pharmaceuticals and personal care products with different anthropogenic pressures in typical watersheds in China.

The Science of the total environment·2024

Related Experiment Video

Updated: Mar 30, 2026

Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module
10:39

Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module

Published on: June 18, 2015

13.9K

CMTM8 is Frequently Downregulated in Multiple Solid Tumors.

Wenjuan Zhang1, Hui Qi, Xiaoning Mo

  • 1*Department of Immunology, School of Basic Medical Sciences, Key Laboratory of Immunology, Ministry of Health, Peking University Health Science Center †National Institutes for Food and Drug Control ‡Peking University People's Hospital, Beijing, P.R. China.

Applied Immunohistochemistry & Molecular Morphology : AIMM
|November 18, 2015
PubMed
Summary
This summary is machine-generated.

CMTM8 is widely expressed in normal human tissues but often lost in solid tumors like liver and lung cancer. This study reveals CMTM8

More Related Videos

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

2.4K
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

7.9K

Related Experiment Videos

Last Updated: Mar 30, 2026

Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module
10:39

Using Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module

Published on: June 18, 2015

13.9K
Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies
07:47

Author Spotlight: Unveiling Transmembrane Protein Family-Related Markers in Gastric Cancer and Implications for Targeted Therapies

Published on: September 15, 2023

2.4K
Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
07:36

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

7.9K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Previous studies suggest CMTM8 influences cell growth, apoptosis, and epithelial-to-mesenchymal transition (EMT).
  • The expression pattern of CMTM8 in normal versus tumorous human tissues remains largely uncharacterized.
  • Understanding CMTM8 expression is crucial for its potential role in tumorigenesis and metastasis.

Purpose of the Study:

  • To extensively analyze the expression of CMTM8 in various normal human tissues.
  • To investigate the expression levels of CMTM8 in multiple types of human solid tumors compared to adjacent normal tissues.
  • To determine the correlation between CMTM8 expression and tumorigenesis.

Main Methods:

  • Real-time quantitative polymerase chain reaction (RT-qPCR) for mRNA expression analysis in normal tissues.
  • High-throughput immunohistochemical (IHC) staining of tissue microarrays (TMAs) to assess protein expression in tumor and normal samples.
  • Statistical analysis (Chi-squared tests) to evaluate the association between CMTM8 expression and tumorigenesis.

Main Results:

  • CMTM8 is broadly expressed across many normal human tissues.
  • CMTM8 expression is frequently downregulated or absent in solid tumors, including liver, lung, colon, rectum, esophagus, and stomach.
  • A significant negative correlation was observed between CMTM8 expression and the presence of tumors in multiple cancer types (P<0.0001 to P<0.01).
  • RT-qPCR confirmed reduced CMTM8 mRNA levels in esophageal carcinomas compared to adjacent normal tissues (P<0.01).

Conclusions:

  • This is the first comprehensive study detailing CMTM8 expression in both normal and cancerous human tissues.
  • The findings strongly support a potential role for CMTM8 as a novel tumor suppressor gene.
  • Downregulation of CMTM8 may be a significant event in the development of various solid tumors.