Related Experiment Video
Updated: Mar 30, 2026
Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation
Published on: January 28, 2018
EFFECT OF SEMEN EXTENDER SUPPLEMENTATION WITH TREHALOSE，VITAMIN C AND E ON POST-THAW MIN PIG SPERM QUALITIES
1Heilongjiang Academy of Agricultural Sciences, Harbin, China. mahong_ok@163.com.
Background:
Cryopreservation of min pig sperm offers an effective method to protect this valuable genetic resource.
Objective:
To determine whether the extender supplementation with some antioxidants is able to improve the quality of post-thawed sperm of min pig.
Materials And Methods:
Ejaculated min pig sperm frozen in lactose-egg yolk (LEY) extender supplemented with trehalose, vitamin C and/or water-soluble vitamin E were thawed, and the quality of sperm samples were evaluated.
Results:
The quality of post-thawed sperm samples was higher in the groups with supplementation than that of the control group (P < 0.05). Furthermore, the addition of 100 mM trehalose and 25 mM water-soluble vitamin E to the freezing extender strongly preserved the quality of the sperm (P < 0.05).
Conclusion:
Trehalose and water-soluble vitamin E in the freezing extender effectively improved post-thaw qualities of min pig sperm.
