Recent Applications for in Vitro Antioxidant Activity Assay.

Area of Science:

• Chemistry
• Biochemistry
• Analytical Chemistry

Background:

• Autoxidation is a significant degradation process.
• Antioxidants play a crucial role in inhibiting autoxidation.
• Understanding antioxidant capacity is vital for various applications.

Purpose of the Study:

• To review recent aspects of antioxidants and inhibited autoxidation.
• To analyze the chemical principles behind antioxidant capacity assays.
• To focus on analytical methods for antioxidant activity determination.

Main Methods:

• Literature review of studies published between 2009 and 2014.
• Analysis of kinetic models for inhibited autoxidation.
• Classification of antioxidant assays based on reaction mechanisms.

Main Results:

• Antioxidant assays are primarily based on hydrogen atom transfer or electron transfer reactions.
• Recent developments in analytical methods for assessing antioxidant activity are highlighted.
• The review synthesizes information on the chemical principles governing these assays.

Conclusions:

• Accurate determination of antioxidant capacity relies on understanding underlying chemical principles.
• The choice of assay method is critical and depends on the reaction mechanism.
• This review provides a focused overview of analytical methods for antioxidant activity assays from 2009-2014.