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[Plasma hemoglobin. Comparison between 2 methods of determination].

M C Khayat¹, F Laforet, F Bureau

¹Laboratoire d'Hématologie, Centre Hospitalier Régional, Caen, France.

Pathologie-Biologie

|March 1, 1989

Summary

Accurate plasmatic hemoglobin measurement is vital for cardiac surgery. Cripps

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Area of Science:

Clinical Chemistry
Biomedical Analysis

Background:

Accurate plasmatic hemoglobin quantification is crucial for cardiac surgery.
Plasmatic hemoglobin levels are significantly lower than whole blood levels, demanding high precision in analytical methods.

Purpose of the Study:

To compare the ammoniacal water method and Cripps' differential method for titrating plasmatic hemoglobin.
To evaluate the performance of these methods against the benzidine method, a recognized reference.

Main Methods:

Comparative analysis of two titration methods: ammoniacal water and Cripps' differential.
Evaluation based on repeatability, limit of detection, accuracy, reference values, and reaction stability.
Correlation studies with the benzidine reference method and between all tested methods.

Main Results:

Cripps' method demonstrated higher accuracy and specificity compared to the ammoniacal water method.
Cripps' method showed closer correlation with the benzidine reference method.
The ammoniacal water method presented challenges in accuracy and ease of use.

Conclusions:

Cripps' differential method is superior for plasmatic hemoglobin titration in cardiac surgery contexts.
The method offers improved simplicity, accuracy, and specificity over the ammoniacal water technique.
Standard solution preparation is a minor challenge for Cripps' method.

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