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Published on: December 7, 2021
JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison
Michael Richter1, Ramon Rosselló-Móra2, Frank Oliver Glöckner3
1Ribocon GmbH, Fahrenheitstraße. 1, 28359 Bremen, Germany.
Unlabelled:
JSpecies Web Server (JSpeciesWS) is a user-friendly online service for in silico calculating the extent of identity between two genomes, a parameter routinely used in the process of polyphasic microbial species circumscription. The service measures the average nucleotide identity (ANI) based on BLAST+ (ANIb) and MUMmer (ANIm), as well as correlation indexes of tetra-nucleotide signatures (Tetra). In addition, it provides a Tetra Correlation Search function, which allows to rapidly compare selected genomes against a continuously updated reference database with currently about 32 000 published whole and draft genome sequences. For comparison, own genomes can be uploaded and references can be selected from the JSpeciesWS reference database. The service indicates whether two genomes share genomic identities above or below the species embracing thresholds, and serves as a fast way to allocate unknown genomes in the frame of the hitherto sequenced species.
Availability And Implementation:
JSpeciesWS is available at http://jspecies.ribohost.com/jspeciesws
Supplementary Information:
Supplementary data are available at Bioinformatics online.
Contact:
mrichter@ribocon.com.
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