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Related Concept Videos

Asepsis01:28

Asepsis

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The condition of being free from disease-causing living pathogens is asepsis. Aseptic techniques include a set of standard practices to achieve asepsis. An example is the regular environmental cleaning of all parts of the healthcare facility and hand hygiene at home before preparing or eating food. Medical and surgical asepsis in healthcare practice protects patients from harmful pathogens, minimizes the risk of contamination of susceptible sites, and reduces the risk of infection transmission.
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Hand hygiene01:23

Hand hygiene

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Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
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Tracheostomy Suctioning II: Procedure01:23

Tracheostomy Suctioning II: Procedure

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Tracheostomy suctioning is a vital nursing procedure that involves removing secretions from the tracheostomy tube to maintain airway patency and prevent respiratory complications. Nurses need to understand the proper technique for tracheostomy suctioning to ensure patient safety and comfort. In this guide, we will outline the step-by-step process for performing tracheostomy suctioning, including preparing the sterile field, donning personal protective equipment (PPE), lubricating and connecting...
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Key Techniques in Microbiology01:19

Key Techniques in Microbiology

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Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
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Tracheostomy Suctioning I: Pre-Procedural Steps01:26

Tracheostomy Suctioning I: Pre-Procedural Steps

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Tracheostomy suctioning is a critical procedure healthcare professionals perform to maintain a patent airway in patients with a tracheostomy tube. This procedure is necessary when secretions accumulate in the airway, causing respiratory distress. Here is a step-wise procedural guide for performing tracheostomy suctioning using an open system.
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First, gather all necessary equipment: a sterile suction catheter, a sterile disposable container, sterile gloves, a towel or...
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Endoscopic Procedures IV: Sigmoidoscopy and Laproscopy01:26

Endoscopic Procedures IV: Sigmoidoscopy and Laproscopy

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Sigmoidoscopy and laparoscopy are distinct medical procedures that enable physicians to internally inspect different parts of the GI tract. Although they serve different purposes, each is essential for diagnosing and, in some cases, treating various medical conditions.
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Related Experiment Video

Updated: Mar 30, 2026

Laparoscopic Pancreatoduodenectomy for Pancreatic Cancer Using In-Situ No-Touch Isolation Technique
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[Aseptic non-touch technique in the operating room: keep it simple].

Solange J A Pans1, Eva Molmans, Susanne C Marczinski

  • 1Ziekenhuis Gelderse Vallei, afd. Anesthesiologie, Ede.

Nederlands Tijdschrift Voor Geneeskunde
|November 19, 2015
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Summary

Bacterial contamination in syringes prepared by anesthesia nurses in the operating room is very low. A modified Aseptic Non-Touch Technique (ANTT) protocol may be suitable for operating room environments, balancing safety and efficiency.

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Area of Science:

  • Medical Microbiology
  • Patient Safety
  • Surgical Nursing

Background:

  • The Aseptic Non-Touch Technique (ANTT) is a protocol for preparing intravenous medications to reduce contamination.
  • ANTT is mandated for all hospital departments, including operating theatres, by the Dutch Healthcare Inspectorate.
  • Operating theatres have unique environmental factors (air treatment, uniforms) compared to nursing wards, necessitating specific evaluation of ANTT.

Purpose of the Study:

  • To determine the bacterial contamination rate of syringes prepared by anesthesia nurses in the operating theatre.
  • To assess the adherence of anesthesia nurses' routine practices to the ANTT protocol.
  • To evaluate the time implications of implementing a strict ANTT protocol in the operating theatre.

Main Methods:

  • A simulation study involving 45 anesthesia nurses preparing 1000 syringes of bacterial culture medium.
  • Bacterial contamination was assessed by culturing syringes for 14 days at 30°C.
  • Nurses' working methods were evaluated via questionnaires, and time investment for strict ANTT was calculated.

Main Results:

  • A low contamination rate of 0.6% (6 syringes) was observed, with normal dermal bacteria identified.
  • No significant difference in working methods was found between nurses who prepared contaminated and uncontaminated syringes.
  • Strict adherence to the ANTT protocol would require an additional 54 minutes per operating theatre daily.

Conclusions:

  • Aseptic preparations in the operating theatre demonstrate very low contamination rates, comparable to those in GMP-certified hospital pharmacies.
  • The findings support the development of a modified ANTT protocol specifically for operating theatre use.
  • A tailored ANTT approach can maintain low contamination rates while potentially optimizing workflow in surgical settings.