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Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast
Published on: December 29, 2023
Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions
Natsumi Maruta1, Yuri Trusov1, Jose R Botella2
1Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, University of Queensland, Brisbane, QLD, 4072, Australia.
Abstract:
Protein-protein interaction studies provide useful insights into biological processes taking place within the living cell. A number of techniques are available to unravel large structural protein complexes, functional protein modules, and temporary protein associations occurring during signal transduction. The choice of method depends on the nature of the proteins and the interaction being studied. Here we present an optimized and simplified yeast three-hybrid method for analysis of protein interactions involving three components.
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