Altered expression of lysosomal hydrolase, acid α-glucosidase, gene in coronary artery disease

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Jingyi Zhang1, Ling Ma, Junping Zhang

  • 1aCollege of Clinical Medicine, Xinxiang Medical University, Xinxiang, Henan bDivision of Cardiology cShandong Provincial Key Laboratory of Cardiac Disease Diagnosis and Treatment, Affiliated Hospital of Jining Medical University, Jining Medical University, Shandong, China.

Coronary Artery Disease
|November 19, 2015
PubMed

Insights

Gene expression of acid α-glucosidase (GAA) is significantly increased in coronary artery disease (CAD) patients. This suggests a potential role for GAA in the development of atherosclerosis and CAD.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cardiovascular Disease Research

Background:

  • Coronary artery disease (CAD) is a complex condition driven by atherosclerosis.
  • Autophagy, a cellular process involving lysosomes, plays a role in lipid and cholesterol metabolism.
  • Previous studies indicated altered expression of autophagic and lysosomal genes in CAD patients.

Purpose of the Study:

  • To investigate the gene expression of acid α-glucosidase (GAA), a lysosomal hydrolase, in patients with CAD.
  • To compare GAA expression levels between CAD patients and ethnically matched controls.

Main Methods:

  • Examined GAA gene expression in large cohorts of CAD patients (n=248) and controls (n=208).
  • Assessed GAA enzymatic activity, protein levels, and transcript levels.
  • Utilized multivariate logistic regression analysis including GAA transcript level, hypertension, diabetes, and smoking.

Main Results:

  • GAA enzymatic activity and transcription levels were significantly elevated in CAD patients compared to controls (P<0.05 and P<0.01, respectively).
  • GAA transcript levels showed a strong association with CAD (odds ratio 5.93, P=3.89×10(-7)).
  • GAA protein levels showed an insignificant increase in CAD patients, potentially due to assay limitations.

Conclusions:

  • GAA gene expression is significantly increased in CAD patients.
  • These findings suggest that GAA may play a role in the pathogenesis of coronary artery disease.
Abstract

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