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The steroid receptor RNA activator protein (SRAP) controls cancer cell migration/motility

Yi Yan1, Charlton Cooper2, Mohammad K Hamedani1

  • 1Manitoba Institute of Cell Biology, 675 McDermot Ave., R3E0V9 Winnipeg, Manitoba, Canada; Department of Biochemistry and Medical Genetics, University of Manitoba, 770 Bannatyne Avenue, R3E0W3 Winnipeg, Manitoba, Canada.

FEBS Letters
|November 20, 2015
PubMed

Insights

The steroid receptor RNA activator gene (SRA1) influences cancer cell motility. SRAP protein overexpression increases cell movement, while SRA/SRAP depletion decreases it, revealing a novel link to SRA1 gene expression.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • The steroid receptor RNA activator gene (SRA1) generates both SRA RNA and SRAP protein.
  • The precise physiological functions of SRA and SRAP are not fully understood.
  • Investigating SRAP's role in cellular processes is crucial for understanding gene regulation.

Purpose of the Study:

  • To identify cellular processes regulated by the SRAP protein.
  • To investigate the impact of SRA1 gene expression on cancer cell behavior.
  • To establish a link between SRA1 and cell motility.

Main Methods:

  • Transcriptome analysis using RNA-sequencing (RNA-seq) in Hela and MDA-MB-231 cancer cells.
  • Depletion of SRA/SRAP transcripts and overexpression of SRAP protein were performed.
  • Live cell imaging was utilized to observe cancer cell motility.

Main Results:

  • RNA-seq and Ontology analyses suggested that SRAP regulates cellular movement.
  • Depletion of SRA/SRAP transcripts resulted in decreased cancer cell motility.
  • Overexpression of SRAP protein led to an increase in cancer cell motility.

Conclusions:

  • This study establishes a novel connection between SRA1 gene expression and cancer cell motility.
  • SRAP protein plays a significant role in regulating the movement of cancer cells.
  • Findings provide new insights into the physiological roles of SRA1 and its protein product.
Keywords:
CancerCell motilityFunctional RNANon-coding RNASteroid receptor RNA activatorSteroid receptor RNA protein

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