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Isolation and Quantification of Axonal mRNAs Using Porous Membrane Inserts and RTddPCR
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The epitranscriptome in modulating spatiotemporal RNA translation in neuronal post-synaptic function.

Shobbir Hussain1, Zafar I Bashir2

  • 1Department of Biology and Biochemistry, University of Bath Bath, UK.

Frontiers in Cellular Neuroscience
|November 20, 2015
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Summary
This summary is machine-generated.

RNA modifications, particularly methylation by NSun2, may control protein synthesis in neurons. This process is linked to synaptic plasticity and involves interactions with FMRP at dendrites.

Keywords:
FMRP (FMR1)Intellectual DisabilityNSun2RNA methylationautism spectrum disordersepitranscriptomeepitranscriptomicssynaptic plasticity

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Area of Science:

  • Molecular Genetics
  • Neuroscience
  • Epitranscriptomics

Background:

  • Next-generation sequencing enables transcriptome-wide investigation of RNA modifications.
  • Epitranscriptomics is an emerging field studying these modifications.
  • RNA modifications dynamically regulate protein synthesis, especially on messenger RNAs.

Purpose of the Study:

  • To investigate the role of RNA modifications in spatiotemporal control of protein synthesis in neurons.
  • To explore the colocalization and interaction of RNA methylase NSun2 and translational repressor FMRP in neuronal dendrites.

Main Methods:

  • Transcriptome-wide analysis of RNA modifications.
  • Immunofluorescence to assess protein colocalization at neuronal dendrites.
  • Analysis of shared mRNA targets between NSun2 and FMRP.

Main Results:

  • NSun2, an RNA methylase, colocalizes with FMRP, a translational repressor, at neuronal dendrites.
  • NSun2 frequently methylates mRNAs encoding postsynaptic proteome components.
  • NSun2 and FMRP share mRNA targets, including those translated in an activity-dependent manner at dendrites.

Conclusions:

  • RNA modifications, regulated by NSun2 and potentially FMRP, are critical for spatiotemporal control of protein synthesis in neurons.
  • These modifications may play a significant role in synaptic plasticity modulation through activity-dependent mechanisms.