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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

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[sgRNA design for the CRISPR/Cas9 system and evaluation of its off-target effects]

Sheng-song Xie1, Yi Zhang2, Li-sheng Zhang1

  • 1Key Lab of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.

Yi Chuan = Hereditas
|November 20, 2015
PubMed

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