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Updated: Jan 18, 2026
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CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
Published on: November 1, 2024
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[Efficient genome editing in human pluripotent stem cells through CRISPR/Cas9]
Gai-gai Liu1, Shuang Li1, Yu-da Wei1
1Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Shanghai 200031, China.
Yi Chuan = Hereditas
|November 20, 2015
Summary
The CRISPR/Cas9 system enables precise genome editing in human stem cells. This technology allows gene disruption, sequence insertion, and large deletions for versatile stem cell research.
Area of Science:
- Molecular Biology
- Genetics
- Stem Cell Biology
Context:
- CRISPR/Cas9 is a powerful RNA-guided genome editing tool.
- Human pluripotent stem cells are crucial for disease modeling and regenerative medicine.
Purpose:
- To demonstrate the versatility of CRISPR/Cas9 technology for genome editing in human pluripotent stem cells.
- To showcase applications including gene disruption, knock-in, and large deletions.
Summary:
- CRISPR/Cas9 was employed to target specific genomic regions in human pluripotent stem cells.
- The system efficiently introduced frameshift mutations, knocked in FLAG tag sequences via homology-directed repair, and induced large deletions using dual-guide multiplexing.
Impact:
- CRISPR/Cas9 offers a versatile platform for stem cell genome editing.
- This technology facilitates functional studies of genes and genomic loci in human pluripotent stem cells.
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