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Updated: Mar 30, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Stereocontrol within polyketide assembly lines
1Department of Molecular Biosciences, The University of Texas at Austin, 2506 Speedway Stop A5000, Austin, TX 78712, USA. adriankc@utexas.edu and Department of Chemistry, The University of Texas at Austin, 105 E 24th St. Stop A5300, Austin, TX 78712, USA.
Abstract:
Most of the stereocenters of polyketide natural products are established during assembly line biosynthesis. The body of knowledge for how stereocenters are set is now large enough to begin constructing physical models of key reactions. Interactions between stereocenter-forming enzymes and polyketide intermediates are examined here at atomic resolution, drawing from the most current structural and functional information of ketosynthases (KSs), ketoreductases (KRs), dehydratases (DHs), enoylreductases (ERs), and related enzymes. While many details remain to be experimentally determined, our understanding of the chemical and physical mechanisms utilized by the chirality-molding enzymes of modular PKSs is rapidly advancing.
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