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Updated: Oct 13, 2026

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay
12:55

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Published on: June 1, 2014

Luciferase cDNA from Japanese firefly, Luciola cruciata: cloning, structure and expression in Escherichia coli

H Tatsumi1, T Masuda, N Kajiyama

  • 1Research and Development Division, Kikkoman Corporation, Noda-city, Chiba, Japan.

Journal of Bioluminescence and Chemiluminescence
|April 1, 1989
PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...