A novel multicomponent redox polymer nanobead based high performance non-enzymatic glucose sensor.

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biosensing

Background:

• Development of sensitive and selective glucose sensors is crucial for diabetes management.
• Non-enzymatic glucose sensors offer advantages over enzyme-based systems, avoiding issues like enzyme instability.
• Graphene and metal nanoparticles are promising materials for electrochemical sensor development due to their unique properties.

Purpose of the Study:

• To fabricate a highly sensitive electrochemical non-enzymatic glucose sensor.
• To utilize a multicomponent nanobead (MCNB) incorporating graphene, ferrocene, and copper nanoparticles.
• To evaluate the sensor's performance, selectivity, and stability for glucose detection.

Main Methods:

• Fabrication of a poly(aniline-co-anthranilic acid)-grafted graphene (G-PANI(COOH)).
• Covalent linking of ferrocene (Fc) to G-PANI(COOH) via polyethylene imine (PEI).
• Electrodeposition of copper nanoparticles (Cu NPs) to form the G-PANI(COOH)-PEI-Fc/Cu-MCNB composite, followed by electrode modification.

Main Results:

• The G-PANI(COOH)-PEI-Fc/Cu-MCNB modified electrode demonstrated excellent electrocatalytic activity for glucose detection.
• The sensor exhibited a wide linear range (0.50–15 mM), low detection limit (0.16 mM), and high sensitivity (14.3 µA mM⁻¹ cm⁻²).
• The sensor showed high selectivity towards glucose, with no response to common interferents and saccharides, and achieved high precision in serum samples.

Conclusions:

• The developed multicomponent nanobead material is highly effective for sensitive and selective non-enzymatic glucose sensing.
• The G-PANI(COOH)-PEI-Fc/Cu-MCNB/GCE sensor demonstrates stability and reliability, comparable to commercial glucose test strips.
• This material presents a promising platform for the development of advanced electrochemical sensors for glucose monitoring in physiological fluids.