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Updated: Mar 30, 2026

Selective Capture of 5-hydroxymethylcytosine from Genomic DNA
Published on: October 5, 2012
Selective oxidation of 5-hydroxymethylcytosine with micelle incarcerated oxidants to determine it at single base
Seketsu Fukuzawa1, Kazuo Tachibana, Shoji Tajima
1Department of Chemistry, School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Abstract:
5-Methylcytosine (5mC) is oxidized by ten-eleven translocation (TET) enzymes. This process followed by thymine DNA glycosylase is proposed to be the mechanism for methylcytosine demethylation. 5-Hydroxymethylcytosine (5hmC) is one of the most important key oxidative metabolites in the demethylation process, and therefore, simple and accurate method to determine 5hmC at single base resolution is desired. In the present study, we developed a mild catalytic oxidation of 5-hmC using micelle incarcerated oxidants that enables to determine the position of 5hmC at single base resolution.
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