CPPsite 2.0: a repository of experimentally validated cell-penetrating peptides

Area of Science:

• Biochemistry
• Molecular Biology
• Drug Delivery

Background:

• Cell-penetrating peptides (CPPs) are crucial for delivering various molecules into cells.
• The previous CPPsite database required updates to reflect recent advancements in CPP research.
• Diverse chemical modifications of CPPs are increasingly utilized to enhance their properties.

Purpose of the Study:

• To present CPPsite 2.0, an updated and expanded manually curated database of CPPs.
• To systematically compile information on chemical modifications and structure-function relationships of CPPs.
• To provide a user-friendly platform for researchers studying CPPs and their applications.

Main Methods:

• Manual curation of peptide entries from recent scientific literature and patents.
• Systematic compilation of data on diverse chemical modifications of CPPs.
• Prediction of tertiary structures for modified and natural residue-containing CPPs using advanced techniques.

Main Results:

• CPPsite 2.0 contains approximately 1850 peptide entries, doubling the previous version's content.
• Detailed information on various chemical modifications (non-natural residues, lipid moieties, etc.) is now systematically included.
• Tertiary structures of CPPs were predicted, and data on in vitro/in vivo model systems and delivered cargoes were maintained.

Conclusions:

• CPPsite 2.0 offers significant improvements in data content and comprehensiveness compared to its predecessor.
• The database facilitates a deeper understanding of CPP structure-function relationships.
• The updated, user-friendly website supports researchers in diverse fields, including drug delivery and molecular biology.