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Association of cytokine gene polymorphisms with hepatitis C virus infection in a population from Rio de Janeiro,

Gustavo Milson Fabrício-Silva1, Bruno Silva Poschetzky2, Renata de Mello Perez2

  • 1Histocompatibility and Cryopreservation Laboratory, Rio de Janeiro State University, Rio de Janeiro, Brazil.

Hepatic Medicine : Evidence and Research
|November 21, 2015
PubMed

Insights

Interleukin-28B (IL-28B) gene variations are linked to spontaneous hepatitis C virus (HCV) clearance and treatment success in Brazilians. The IL-6 (-174) C allele may also aid spontaneous recovery and reduce liver inflammation.

Area of Science:

  • Immunogenetics
  • Hepatology
  • Virology

Background:

  • Host immune response significantly impacts Hepatitis C Virus (HCV) infection outcomes and disease progression.
  • Investigating cytokine gene polymorphisms offers insights into susceptibility to persistent HCV infection versus spontaneous viral clearance.

Purpose of the Study:

  • To explore cytokine gene polymorphisms as potential markers for HCV infection susceptibility or spontaneous clearance.
  • To analyze these genetic factors in a Brazilian population from Rio de Janeiro.

Main Methods:

  • Genotyping of cytokine polymorphisms including TNF-alpha, TGF-beta 1, IL-10, IL-6, and IFN-gamma using PCR.
  • Assessing IL-28B polymorphisms (rs12979860, rs12980275, rs8099917) via real-time PCR.
  • Study included 245 chronic hepatitis C patients, 41 spontaneous recovery patients, and 189 healthy controls.

Main Results:

  • IL-28B rs12979860 CC and rs12980275 AA genotypes strongly correlated with spontaneous HCV recovery and treatment response.
  • The IL-6 (-174) C allele showed a protective association against chronic hepatitis C (CHC) and was more prevalent in spontaneous recovery patients.
  • Multivariate analysis identified male sex, specific IL-28B genotypes (CT, TT), and TGF-beta 1 codon 10 TC genotype as factors associated with CHC.

Conclusions:

  • IL-28B polymorphisms are key determinants of spontaneous HCV clearance and therapeutic response in the studied Brazilian cohort.
  • The IL-6 (-174) C allele appears to play a role in spontaneous recovery and may contribute to reduced liver inflammation.
Abstract

Keywords:
HCV clearanceSNPinflammationinterleukin-28interleukin-6

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