Related Experiment Video

Updated: Mar 30, 2026

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model
05:03

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model

Published on: December 14, 2016

9.1K

Biofilm Formation and Virulence Factors Among Pseudomonas aeruginosa Isolated From Burn Patients

Zahra Ghanbarzadeh Corehtash1, Ahmad Khorshidi1, Farzaneh Firoozeh1

  • 1Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, IR Iran.

Jundishapur Journal of Microbiology
|November 21, 2015
PubMed
Abstract

Insights

Multidrug-resistant Pseudomonas aeruginosa (MDRPA) is a significant threat in burn wound infections. High rates of antibiotic resistance and biofilm formation were observed, highlighting the urgent need for effective treatment strategies.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Public Health

Background:

  • Pseudomonas aeruginosa is a common cause of burn wound infections.
  • Multidrug-resistant P. aeruginosa (MDRPA) poses a significant public health challenge.
  • Virulence factors like nan1 and exoA contribute to P. aeruginosa pathogenicity.

Purpose of the Study:

  • To investigate antibiotic resistance patterns in P. aeruginosa from burn patients.
  • To determine the prevalence of MDRPA and biofilm formation.
  • To assess the occurrence of nan1 and exoA virulence genes.

Main Methods:

  • 144 P. aeruginosa isolates were collected from burn patients.
  • Antibiotic susceptibility testing was performed using agar disk diffusion.
  • Biofilm formation was assessed via crystal violet microtiter plate assay.
  • Polymerase chain reaction (PCR) was used to detect exoA and nan1 genes.

Main Results:

  • High resistance rates were observed for ciprofloxacin (93.7%), aztreonam (86.8%), and imipenem (79.2%).
  • 93.1% of isolates were identified as MDRPA, with 92.4% exhibiting biofilm formation.
  • The exoA gene was present in 75% of isolates, and nan1 in 11.8%.

Conclusions:

  • The high prevalence of MDRPA and biofilm-producing strains is a critical public health concern.
  • Biofilm production was significantly higher in MDRPA isolates compared to non-MDRPA isolates (P < 0.001).
  • Findings underscore the need for enhanced infection control and therapeutic strategies against MDRPA in burn patients.
Keywords:
BiofilmsBurnsPseudomonas aeruginosaVirulence factors

More Related Videos

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation07:16

Author Spotlight: Studying Host-Microbe Interactions in Wound Biofilm Formation

Published on: June 16, 2023

2.6K
A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection06:56

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection

Published on: February 20, 2020

9.3K

Related Concept Videos

Biofilms01:29

Biofilms

Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.9K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
878
Colonisation of Pathogens01:25

Colonisation of Pathogens

Pathogen colonization of host tissues is a critical step in the development of infectious diseases. Various pathogenic microorganisms, including bacteria, fungi, viruses, and protozoa, have evolved complex strategies to attach to, invade, and persist within host environments. These mechanisms enable pathogens to establish infections, evade immune responses, and resist antimicrobial treatments.Attachment to Host CellsIn bacteria, colonization typically begins with adherence to host epithelial...
34
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
62