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Updated: Mar 30, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Noncontiguous finished genome sequence and description of Necropsobacter massiliensis sp. nov
C I Lo1, R Padhamanabhan2, B Fall3
1Faculté de médecine, URMITE, UM63, CNRS 7278, IRD 198, Inserm U1095, Aix-Marseille Université, Marseille, France ; Campus International UCAD-IRD, Senegal.
Abstract:
Strain FF6(T) was isolated from the cervical abscess of a 4-year-old Senegalese boy, in Dakar, Senegal. MALDI-TOF MS did not provide any identification. This strain exhibited a 95.17% 16S rRNA sequence identity with Necropsobacter rosorum. Using a polyphasic study including phenotypic and genomic analyses, strain FF6(T) was an aero-anaerobic Gram-negative cocobacillus, oxidase positive, and exhibited a genome of 2,493,927 bp (1 chromosome but no plasmid) with a G+C content of 46.2% that coded 2,309 protein-coding and 53 RNA genes. On the basis of these data, we propose the creation of Necropsobacter massiliensis sp. nov.
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