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Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...

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Related Experiment Video

Updated: Mar 30, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Automated analysis of single cells using Laser Tweezers Raman Spectroscopy.

S Casabella¹, P Scully², N Goddard³

¹Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, M1 7DN, UK. peter.gardner@manchester.ac.uk and The Photon Science Institute, The University of Manchester, Oxford Road, Manchester, M13 9PL, UK.

|November 21, 2015

Summary

This summary is machine-generated.

This study introduces an automated microfluidic system for Laser Tweezers Raman Spectroscopy (LTRS) to detect and differentiate cancer cells. This innovation reduces manual labor and enables efficient, real-time analysis of cell lines.

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An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

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Published on: January 9, 2020

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Published on: September 4, 2010

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Last Updated: Mar 30, 2026

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

An Integrated Raman Spectroscopy and Mass Spectrometry Platform to Study Single-Cell Drug Uptake, Metabolism, and Effects

Published on: January 9, 2020

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Direct Analysis of Single Cells by Mass Spectrometry at Atmospheric Pressure

Published on: September 4, 2010

Area of Science:

Biomedical Engineering
Spectroscopy
Cell Biology

Background:

Label-free detection of individual cancer cells using Laser Tweezers Raman Spectroscopy (LTRS) has advanced significantly.
Current LTRS methods often rely on manual cell trapping, which is labor-intensive and limits throughput.
Automated analysis is crucial for efficient and reproducible LTRS of live cells.

Purpose of the Study:

To develop and demonstrate a low-cost microfluidic flow chamber for automated single-cell trapping and analysis using LTRS.
To compare a single-channel and a dual-channel microfluidic device for LTRS applications.
To evaluate the discrimination of live prostate epithelial cells and lymphocytes using the automated system.

Main Methods:

Introduction of a novel microfluidic flow chamber for optical trapping of single cells.
Implementation of two microfluidic designs: a basic single-channel device and an advanced dual-channel device.
Application of Laser Tweezers Raman Spectroscopy (LTRS) for label-free analysis of trapped cells.

Main Results:

The microfluidic system enables automated, single-cell trapping and LTRS analysis, reducing operator dependency.
The dual-channel device facilitates automated capture and analysis of multiple cell lines without manual intervention.
Successful discrimination between live epithelial prostate cells and lymphocytes was achieved.

Conclusions:

The developed microfluidic flow chamber offers a simple, low-cost solution for automated LTRS.
This automated approach significantly enhances the efficiency and reduces the operator input required for single-cell analysis.
The system demonstrates potential for improved cancer cell detection and discrimination compared to traditional batch analysis methods.