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Area of Science:

  • Oncology
  • Biophysics
  • Biochemistry

Background:

  • Head and neck squamous cell cancer (HNSCC) is a prevalent malignancy with limited therapeutic advancements.
  • Current treatments, including monoclonal antibodies, have not significantly improved patient prognosis.
  • Cold atmospheric plasma (CAP) is an emerging technology with potential for cancer therapy.

Purpose of the Study:

  • To evaluate the efficacy of a surface micro-discharging (SMD) plasma device against HNSCC cell lines.
  • To assess the impact of CAP on cancer cell viability, DNA integrity, and apoptosis.
  • To determine the potential of SMD plasma as a novel cancer treatment modality.

Main Methods:

  • Utilized MTT assay to measure cell viability.
  • Employed alkaline microgel electrophoresis (comet assay) to quantify DNA fragmentation.
  • Performed Annexin-V/PI staining to detect apoptosis induction.
  • Tested two HNSCC cell lines with varying CAP treatment durations (30-180 seconds).

Main Results:

  • CAP treatment significantly reduced HNSCC cell viability, with IC50 reached within 120 seconds.
  • Comet assay indicated dose-dependent DNA fragmentation, a key factor in CAP's anti-cancer effect.
  • Annexin-V/PI staining confirmed CAP-induced apoptosis in HNSCC cells, though dose dependency was not significant.

Conclusions:

  • SMD plasma technology demonstrates significant anti-cancer effects on HNSCC cell lines.
  • CAP effectively reduces cell viability and induces DNA fragmentation and apoptosis.
  • SMD plasma represents a promising new approach for head and neck cancer treatment.