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Isolation of Polyvalent Bacteriophages by Sequential Multiple-Host Approaches
Pingfeng Yu1, Jacques Mathieu2, Mengyan Li1
1Department of Civil and Environmental Engineering, Rice University, Houston, Texas, USA.
Applied and Environmental Microbiology
|November 22, 2015
Summary
Broad-host-range phages, often overlooked, can be effectively isolated using sequential multihost methods. This approach aids in studying their ecological roles and developing new applications for these versatile viruses.
Area of Science:
- Microbiology
- Virology
- Environmental Science
Background:
- Phage isolation methods often favor narrow-host-range phages, neglecting broad-host-range (polyvalent) phages.
- The ecological significance of polyvalent phages remains largely unexplored due to isolation biases.
Purpose of the Study:
- To develop and evaluate sequential multihost isolation methods for broad-host-range phages.
- To assess the infectivity and characterize polyvalent phages for potential applications.
Main Methods:
- Developed two sequential multihost isolation techniques.
- Tested both culture-dependent and culture-independent phage libraries for broad infectivity.
- Characterized two isolated polyvalent phages (PX1 and PEf1) regarding adsorption, latent time, and burst size.
Main Results:
- Isolated lytic phages exhibited interspecies or interorder infectivity with high plating efficiency.
- Polyvalent phages showed efficient adsorption, short latent periods, and significant burst sizes across different hosts.
- Enriched polyvalent phages demonstrated >60% lethality against Pseudomonas aeruginosa and ~50% against Pseudomonas syringae.
Conclusions:
- Sequential multihost isolation is effective for obtaining environmentally abundant polyvalent phages.
- This method facilitates the study of polyvalent phage ecology and their potential in novel applications, such as biocontrol.
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