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Differentiation and Characterization of Neural Progenitors and Neurons from Mouse Embryonic Stem Cells
Published on: May 15, 2020
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Immunophenotype of mouse cerebral hemispheres-derived neural precursor cells
Kyriaki-Nefeli Poulatsidou1, Roza Lagoudaki1, Olga Touloumi1
1Laboratory of Experimental Neurology and Neuroimmunology, B' Department of Neurology, AHEPA University Hospital Aristotle University, Thessaloniki 54636, Greece.
Neuroscience Letters
|November 22, 2015
Summary
Postnatally isolated neural precursor cells (piNPCs) show promise for treating Experimental Autoimmune Encephalomyelitis (EAE). These cells integrate into host tissue and improve symptoms, with a defined immunophenotype aiding transplantation development.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Experimental Autoimmune Encephalomyelitis (EAE) is a model for multiple sclerosis.
- Neural precursor cells (NPCs) are being explored for cell-based therapies.
- Postnatally isolated neural precursor cells (piNPCs) from mouse cerebral tissue have shown therapeutic potential in EAE models.
Purpose of the Study:
- To characterize the immunophenotype of mouse cerebral piNPCs.
- To identify specific cell surface markers for piNPC identification.
- To assess the utility of piNPCs in developing transplantation protocols for EAE.
Main Methods:
- Isolation and in vitro culture of mouse cerebral piNPCs.
- Formation of neurospheres by piNPCs.
- Flow cytometry analysis to determine piNPC immunophenotype.
Main Results:
- Mouse cerebral piNPCs formed neurospheres expressing polysialylated neural adhesion molecule (PSA-NCAM).
- piNPCs differentiated primarily into glial cells, with some neuronal differentiation.
- Flow cytometry revealed piNPCs are positive for CD24, CD44, and CD133, but negative for CD15, CD184, and CD49d.
Conclusions:
- The identified immunophenotype (CD24+, CD44+, CD133+) provides a novel method for characterizing neonatal mouse cerebral piNPCs.
- This characterization is crucial for developing standardized transplantation protocols for EAE therapy.
- piNPCs demonstrate potential for cell-based therapies in neuroinflammatory diseases.
