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Updated: Mar 29, 2026

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope
Published on: February 28, 2019
Shapes of dominant transition paths from single-molecule force spectroscopy
1Department of Chemistry and Institute for Computational Engineering and Sciences, University of Texas at Austin, Austin, Texas 78712, USA.
Abstract:
Recent single molecule measurements of biomolecular folding achieved the time resolution sufficient for observation of individual transition paths. This note discusses several ways in which transition path ensembles can be statistically analyzed to extract a single, "typical" transition path. Analytical approximations derived here for such a transition path further allow one to estimate dynamical parameters (such as the intramolecular diffusion coefficient) directly from the transition path shapes.
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