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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection
Published on: May 24, 2020
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Rabbit CD200R binds host CD200 but not CD200-like proteins from poxviruses.
Munir Akkaya1, Lai-Shan Kwong1, Erdem Akkaya1
1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
Virology
|November 23, 2015
Summary
Myxoma virus M141 protein, a poxvirus CD200-like molecule, modulates the rabbit immune system. This study found M141 inhibits immune cells without binding to the CD200 receptor (CD200R).
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- CD200 is an immune-inhibitory protein recognized by its receptor, CD200R, on myeloid cells.
- Herpesviruses utilize CD200 to suppress host immune responses.
- Poxviruses may possess CD200-like proteins, but their function and interaction with CD200R are unclear.
Purpose of the Study:
- To characterize rabbit CD200 and CD200R.
- To investigate if myxoma virus M141 protein, a putative CD200 orthologue, binds to rabbit CD200R.
- To determine if M141's immunomodulatory effects are mediated through CD200R.
Main Methods:
- Recombinant protein expression and binding assays.
- Cell-based expression of full-length proteins.
- Myxoma virus infection of cells.
- RNA interference (knockdown) to reduce CD200R expression.
Main Results:
- No binding was detected between M141 and CD200R using various experimental setups.
- M141 exhibited immunomodulatory effects on RAW 264.7 cells during myxoma virus infection.
- Knockdown of CD200R did not abolish the inhibitory effect of M141.
Conclusions:
- Rabbit poxvirus CD200-like proteins, including M141, can cause significant immunomodulation.
- These viral CD200-like proteins achieve immune modulation independently of the host CD200R pathway.
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