Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent
Guangqian Lan1, Bitao Lu2, Tianyou Wang2
1College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
Colloids and Surfaces. B, Biointerfaces
|November 23, 2015
Summary
A novel chitosan and gelatin composite (CG) effectively stops bleeding, showing superior hemostatic properties in animal models. This absorbable material is non-toxic and promotes tissue regeneration, offering a promising solution for wound healing.
Area of Science:
- Biomaterials Science
- Hemostasis Research
- Tissue Engineering
Background:
- Chitosan is recognized for its hemostatic capabilities.
- Developing effective hemostatic agents is crucial for managing bleeding.
- Silkworm pupae offer a sustainable source for chitosan extraction.
Purpose of the Study:
- To evaluate the hemostatic efficacy of a chitosan-gelatin composite (CG).
- To investigate the properties of CG derived from silkworm pupae and gelatin.
- To assess the biocompatibility and degradation of the CG material.
Main Methods:
- Fabrication of chitosan/gelatin sponges (CG) with varying ratios, cross-linked with tannins and freeze-dried.
- In vitro assessment of blood-clotting index (BCI).
- In vivo evaluation in rabbit artery bleeding and liver models, alongside cell toxicity and subcutaneous implantation tests.
Main Results:
- The optimal CG ratio of 5/5 (W/W) demonstrated the highest BCI in vitro.
- CG exhibited superior hemostatic effects compared to chitosan or gelatin alone in animal models.
- No significant cytotoxicity was observed, and CG showed complete degradation with vascularization within 6 weeks post-implantation.
Conclusions:
- The chitosan-gelatin composite (CG) is an effective and absorbable hemostatic material.
- CG's efficacy is attributed to its platelet absorption and liquid adsorption capabilities.
- CG is biocompatible, non-toxic, and promotes tissue regeneration, indicating its potential clinical applications.
