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Updated: Mar 29, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
[Measurement and analysis of oxidative stress-related elements in blood components of chronic unpredictable mild
Objective:
To study the effects of chronic unpredictable mild stress (CUMS) on the concentrations and distribution of elements related to oxidative stress in plasma and erythrocyte.
Methods:
The rats were randomly divided into two groups, CUMS group and the control. The CUMS group received 28 days stress. Then 17 kinds of elements in plasma and erythrocyte were detected and the correlation ships between elements were analysed, then make comparison between two groups.
Results:
The concentrations of Mo and B in plasma and Se in erythrocyte were higher in CUMS group than control (P <0. 05). The levels of Sn, Cr, Th and T1 in plasma as well as Mn and Pb in erythrocyte were lower in CUMS group (P < 0. 05). The strength and direction of correlation ships between elements were changed in CUMS group. There had a negative correlation with Fe in plasma in control (r = - 0. 581, P = 0. 018), but a positive correlation in CUMS (r = 0. 473, P = 0. 035), the same with Th-Mg (control: r = - 0. 610, P = 0. 012; CUMS: r = 0. 596, P = 0. 006).
Conclusion:
Concentrations and distribution of elements related to oxidative stress are changedby CUMS, most of the elements increased are related to anti-oxidative stress, while the reduced elements are prooxidative stress.
