Related Experiment Video
Updated: Mar 29, 2026

An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
Published on: July 31, 2019
In vitro fermentation of juçara pulp (Euterpe edulis) by human colonic microbiota
Karla Bigetti Guergoletto1, Adele Costabile2, Gema Flores3
1Department of Food and Nutrition Sciences, The University of Reading, RG6 6AP Reading, UK; Department of Food Science and Technology, State University of Londrina, Celso Garcia Cid Road, Km 380, C.P.:6001, 86051-970 Londrina, Brazil.
Abstract:
This study was carried out to investigate the potential fermentation properties of juçara pulp, using pH-controlled anaerobic batch cultures reflective of the distal region of the human large intestine. Effects upon major groups of the microbiota were monitored over 24h incubations by fluorescence in situ hybridisation (FISH). Short-chain fatty acids (SCFA) were measured by HPLC. Phenolic compounds, during an in vitro simulated digestion and fermentation, were also analysed. Juçara pulp can modulate the intestinal microbiota in vitro, promoting changes in the relevant microbial populations and shifts in the production of SCFA. Fermentation of juçara pulp resulted in a significant increase in numbers of bifidobacteria after a 24h fermentation compared to a negative control. After in vitro digestion, 46% of total phenolic content still remained. This is the first study reporting the potential prebiotic effect of juçara pulp; however, human studies are necessary to prove its efficacy.
Related Concept Videos
Microbial Fermentation
Microbiota of the Large Intestine
Microbes in the Production of Fermented Foods
Bacterial Flora of the Large Intestine
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
Development of Human Microbiota
Microbiota of the Stomach and Small Intestine

