Multifunctional nano manganese ferrite ferrofluid for efficient theranostic application.

Area of Science:

• Nanotechnology
• Biomedical Engineering
• Materials Science

Background:

• Superparamagnetic iron oxide nanoparticles (SPIONs) are utilized in biomedical applications.
• Enhancing SPIONs' properties for theranostics requires careful material design and surface modification.

Purpose of the Study:

• To synthesize and characterize manganese-substituted iron oxide nanoparticles (MnIOTCs) with trisodium citrate coating.
• To evaluate MnIOTCs as negative magnetic resonance imaging (MRI) contrast agents and for hyperthermia treatment.
• To assess the theranostic potential of MnIOTCs in cancer therapy.

Main Methods:

• Synthesis of MnIOTCs with controlled Mn(2+) to Fe(2+) molar ratio (3:1) and trisodium citrate coating.
• Physicochemical characterization using XRD, TEM, FTIR, TGA, DLS, zeta potential, and vibrating sample magnetometry.
• In vitro evaluation of MRI contrast enhancement (T2 relaxivity) and hyperthermia-induced apoptosis in HeLa cells (FACS, cLSM, E-SEM).
• Biocompatibility assessment via alamar blue assay and hemolysis studies.

Main Results:

• Synthesized MnIOTCs exhibited superparamagnetic properties and high T2 relaxivity (184.6 mM(-1)s(-1)).
• Application of alternating magnetic field to MnIOTCs induced apoptosis in HeLa cells, with significant morphological disruption.
• Biocompatibility studies confirmed good cytocompatibility and hemocompatibility of the synthesized nanoparticles.

Conclusions:

• MnIOTCs demonstrate significant potential as dual-modal theranostic agents.
• The developed MnIOTCs function effectively as negative MRI contrast agents and therapeutic hyperthermia agents.
• These findings support the utility of MnIOTCs in the field of theranostics for cancer treatment.