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Updated: Mar 29, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

Conserved mRNA-binding proteomes in eukaryotic organisms.

Ana M Matia-González¹, Emma E Laing¹, André P Gerber¹

¹Department of Microbial and Cellular Sciences, School of Biosciences and Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, UK.

Nature Structural & Molecular Biology

|November 24, 2015

Summary

This summary is machine-generated.

Researchers identified hundreds of RNA-binding proteins (RBPs) interacting with messenger RNAs (mRNAs) in yeast and worms. Many of these proteins, crucial for gene regulation, are evolutionarily conserved, suggesting ancient roles in coordinating cellular processes.

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Area of Science:

Molecular Biology
Genetics
Evolutionary Biology

Background:

RNA-binding proteins (RBPs) are critical regulators of gene expression at the post-transcriptional level.
While high-throughput methods have identified many RBPs, a comprehensive understanding of their roles in vivo remains incomplete.
Identifying the full spectrum of proteins interacting with messenger RNA (mRNA) is essential for understanding cellular regulation.

Purpose of the Study:

To comprehensively identify proteins that interact with polyadenylated mRNAs in Saccharomyces cerevisiae and Caenorhabditis elegans.
To investigate the dynamic changes in mRNA-binding protein complexes during apoptosis in C. elegans.
To explore the evolutionary conservation and potential ancient functions of these mRNA-binding proteins.

Main Methods:

Utilized high-throughput screening to identify proteins reproducibly interacting with polyadenylated mRNAs in yeast and C. elegans.
Analyzed differential association of mRNA-binding proteins (mRPBs) in C. elegans larvae undergoing apoptosis.
Comparative analysis of identified mRBPomes between yeast and C. elegans.

Main Results:

Identified 765 and 594 proteins interacting with polyadenylated mRNAs in Saccharomyces cerevisiae and Caenorhabditis elegans, respectively.
Observed differential association of mRPBs during induced apoptosis in C. elegans.
Found significant evolutionary conservation of mRBPome components, including metabolic and proteasomal proteins, between yeast and C. elegans.

Conclusions:

The study provides a comprehensive catalog of mRNA-interacting proteins in two model organisms.
Evolutionarily conserved mRNA-binding proteins suggest ancient regulatory mechanisms.
Hypothesized that enzyme-mRNA interactions represent an ancient mechanism for post-transcriptional coordination of metabolic pathways, potentially originating from the 'RNA world'.