Glucose-induced insulin secretion from perifused isolated islets in rats

Glucose-induced insulin secretion in rat pancreas islets was studied by means of the modified perifusion technique. The biphasic insulin increase after 0.3% glucose application consisted of a rapid response (the first phase response) and a delayed one (the second phase response). The former was brought about by shortening the 0.3% glucose application time to one and a half minutes and the latter by treatment with 1 microgram/ml of synthetic somatostatin. It was proposed that the rapid response depended mainly on D-cell activity and the delayed response depended on B-cell function.