Streptomyces spongiicola sp. nov., an actinomycete derived from marine sponge

A novel actinomycete (strain HNM0071^T) was isolated from an unidentified marine sponge collected from the coast of Sanya City, PR China and its taxonomic position was investigated. The major menaquinones were MK-9 (H₆) (65.6 %), MK-9 (H₄) (23.8 %) and MK-9 (H₈) (10.6 %). The predominant fatty acids were anteiso-C_15 : 0 (25.5 %), iso-C_16 : 0 (19.5 %) and iso-C_15 : 0 (15.4 %). The predominant phospholipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, four unidentified phospholipids were found. The G+C content of the genomic DNA was 69.8 mol%. Based on 16S rRNA gene sequence analysis, strain HNM0071^T was most closely related to Streptomyces wuyuanensis FX61^T (97.3 %). The 16S rRNA gene sequence similarity to all other species of the genus Streptomyces was less than 97.0 %. DNA-DNA hybridization between strain HNM0071^T and its closest phylogenetic relative, Streptomyces wuyuanensis FX61^T, showed 25.0 % relatedness. Based on phenotypic and genotypic characteristics, strain HNM0071^T represents a novel species of the genus Streptomyces whose name is proposed as Streptomyes spongiicola sp. nov. The type strain is HNM0071^T ( = CCTCCAA2015018^T = KCTC 39604^T).