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Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
Published on: December 17, 2015
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Varying hemin concentrations affect Porphyromonas gingivalis strains differently
Manabu Ohya1, Marni E Cueno2, Muneaki Tamura3
1Division of Oral Health Science, Nihon University Graduate School of Dentistry, Tokyo 101-8310 Japan; Department of Microbiology, Nihon University School of Dentistry, Tokyo 101-8310 Japan.
Microbial Pathogenesis
|November 25, 2015
Summary
Heme concentration fluctuations impact Porphyromonas gingivalis strains differently. This study reveals varying Arg-gingipain, H2O2, and SOD levels across strains, highlighting strain-specific responses to heme availability.
Area of Science:
- Microbiology
- Periodontal disease research
Background:
- Porphyromonas gingivalis is a key pathogen in periodontal disease.
- Heme is essential for P. gingivalis growth, but its availability varies in periodontal pockets.
- Previous studies have not fully elucidated how heme concentration fluctuations affect different P. gingivalis strains.
Purpose of the Study:
- To investigate the effects of varying hemin concentrations on representative P. gingivalis strains.
- To analyze the impact of heme availability on Arg-gingipain (Rgp) activity, hydrogen peroxide (H2O2), and superoxide dismutase (SOD) levels.
- To determine strain-specific responses of P. gingivalis to fluctuating heme concentrations.
Main Methods:
- Cultured six representative P. gingivalis strains (FDC381, MPWIb-01, TDC60, ATCC49417, W83, HNA99) in media with varying hemin concentrations (5x, 1x, 0.5x, 0.1x) for 24 hours.
- Lysed bacterial samples and standardized protein content.
- Measured levels of Arg-gingipain (Rgp), H2O2, and SOD in the analyzed strains (excluding MPWIb-01 and HNA99 due to study focus).
Main Results:
- Rgp activity showed strain-specific peaks at different hemin concentrations: 1x for FDC381, 5x for TDC60, 0.5x for ATCC49417, and both 5x and 0.5x for W83.
- FDC381 exhibited consistent H2O2 levels across all hemin concentrations, with varying SOD levels.
- TDC60 showed lowest H2O2 at 1x hemin, with increased SOD relative to hemin concentration. ATCC49417 had stable H2O2 levels and higher SOD at 1x and 0.5x hemin. W83 displayed similar H2O2 and SOD levels irrespective of hemin concentration.
Conclusions:
- Hemin concentration significantly influences P. gingivalis virulence factors like Rgp, H2O2, and SOD in a strain-dependent manner.
- Understanding these strain-specific responses to heme variability is crucial for comprehending P. gingivalis's adaptation and persistence in periodontal environments.
- The findings underscore the complex interplay between nutrient availability and bacterial physiology in the pathogenesis of periodontal disease.
