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Updated: Mar 29, 2026
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Published on: August 29, 2022
[The effect of estrogen on adipogenic differentiation of rat bone mesenchymal stem cells]
Jie Wang1, Ning-juan Ouyang, Peng Zhang
1Department of Oral and Craniomaxillofacial Science, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Lab of Stomatology. Shanghai 200011, China.E-mail:jadewong19881113@hotmail.com.
Purpose:
In this study, 10⁻⁹ mol/L 17 β-estradiol (E2) was applied in the adipogenic differentiation of rat bone mesenchymal stem cells (rBMSCs) and the effect of E2 was explored.
Methods:
Rat BMSCs were obtained from the femurs and tibias of SD rats. 10⁻⁹ mol/L E2 was involved in the adipogenic differentiation of rBMSCs. Oil red staining, real time PCR and Western blot were carried out to detect the effect of 10⁻⁹ mol/L E2 on adipogenic differentiation of rBMSCs. The data was statistically analyzed using SPSS 19.0 software package.
Results:
The use of 10⁻⁹ mol/L E2 decreased the amount of lipid droplets in rBMSCs and weakened the expression of adipogenic related genes and proteins like C/EBP α, C/EBP β, PPAR γ, aP2, and ARDP, which were significantly lower than the adipogenic induced group.
Conclusions:
The use of 10⁻⁹ mol/L E2 inhibited adipogenic differentiation of rBMSCs significantly in vitro.
