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Related Experiment Videos

Urinary LDH-release for evaluation of postischemic renal function.

G Kehrer1, M Blech, M Kallerhoff

  • 1Zentrum Physiologie and Pathophysiologie der Universität Göttingen, FRG.

Klinische Wochenschrift
|May 2, 1989
PubMed
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Urinary enzyme levels, specifically lactate dehydrogenase (LDH), can help assess kidney injury after ischemia. Adjusting LDH levels for glomerular filtration rate (GFR) improves accuracy in evaluating renal damage.

Area of Science:

  • Nephrology
  • Surgical Research
  • Biochemistry

Background:

  • Renal ischemia can lead to tubular injury, affecting enzyme transport into urine.
  • Urinary enzyme levels are influenced by tubular flow and washout, complicating direct assessment.
  • Evaluating early post-ischemic renal damage requires methods that account for changing physiological conditions.

Purpose of the Study:

  • To investigate the utility of urinary enzyme determinations in assessing early renal ischemic injury.
  • To determine if adjusting urinary enzyme concentrations for glomerular filtration rate (GFR) improves injury assessment.
  • To compare the effectiveness of different protective solutions in mitigating renal ischemic damage.

Main Methods:

  • Canine kidneys were subjected to ischemia after perfusion with various protective solutions.

Related Experiment Videos

  • During reperfusion, endogenous creatinine clearance, urine volume, and urinary lactate dehydrogenase (LDH) concentration were measured.
  • Urinary LDH levels were analyzed directly and adjusted for GFR.

    • Main Results:

    • Urinary LDH concentration alone provided only a rough estimate of renal ischemic damage.
    • Standardizing urinary LDH by GFR significantly improved the ability to grade ischemic stress.
    • Adding L-aspartate to the HTK solution, replacing chloride, resulted in lower average urinary LDH release.

    Conclusions:

    • Urinary enzyme release, when standardized to GFR, can be a valuable diagnostic tool for assessing early post-ischemic renal injury.
    • The choice of protective solution, such as using L-aspartate in HTK, may influence the degree of renal protection.
    • This method offers a more refined approach to evaluating renal recovery after protective interventions.