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Dose Titration of Walleye Dermal Sarcoma (WDS) Tumor Filtrate
R G Getchell1, G A Wooster1, C A Sutton1
1a Aquatic Animal Health Program, Department of Microbiology and Immunology , College of Veterinary Medicine, Cornell University , Ithaca , New York , 14853 , USA.
Journal of Aquatic Animal Health
|November 25, 2015
Summary
Researchers determined the minimum dose of walleye dermal sarcoma virus required to induce tumors. Approximately 10(7) viral RNA copies applied topically were sufficient to cause walleye tumors.
Area of Science:
- Ichthyology
- Virology
- Oncology
Background:
- Walleye dermal sarcoma is a tumor affecting fish.
- The causative agent is a virus within dermal sarcoma tumor filtrates.
Purpose of the Study:
- To determine the minimum infectious dose of walleye dermal sarcoma virus.
- To establish a quantitative relationship between viral load and tumor induction.
Main Methods:
- Topical application of a 10-fold dilution series of cell-free tumor filtrates to walleyes.
- Incubation at 15°C for 20 weeks to observe tumor development.
- Quantification of viral RNA copies using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
Main Results:
- The initial inoculum contained approximately 10(10) viral RNA copies per 100 μL.
- A 1,000-fold dilution of the inoculum, equivalent to approximately 10(7) viral RNA copies, was the minimum dose to induce tumors.
- Tumor induction was observed after 20 weeks of incubation.
Conclusions:
- The study quantifies the infectious dose of walleye dermal sarcoma virus.
- Topical application is an effective method for challenging fish with the virus.
- Understanding the viral dose is crucial for studying tumor pathogenesis in walleyes.
