Binding-regulated click ligation for selective detection of proteins

Area of Science:

• Biochemistry
• Molecular Biology
• Chemical Biology

Background:

• Protein detection is crucial in diagnostics and research.
• Existing methods may lack selectivity or require complex procedures.
• Integrating DNA-based sensing with specific molecular recognition is desirable.

Purpose of the Study:

• To develop a novel sensing strategy for selective protein detection.
• To leverage click chemistry for probing small-molecule ligand-protein interactions.
• To create a versatile platform for protein sensing applicable to various targets.

Main Methods:

• Development of a binding-regulated click ligation (BRCL) strategy.
• Utilizing small-molecule ligands and clickable DNA probes.
• Verification via agarose gel electrophoresis, electrochemical impedance spectroscopy, and fluorescence assays.

Main Results:

• The BRCL strategy demonstrated feasibility and effectiveness in protein detection.
• The assay exhibited desirable selectivity due to specific ligand-target recognition.
• The method was validated for different proteins (avidin, folate receptor) and in complex biological samples.

Conclusions:

• The BRCL strategy provides a novel and effective method for selective protein detection.
• This approach integrates click chemistry with DNA-based sensing for protein analysis.
• The developed sensing method shows general applicability and potential for future diagnostics.