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Infection of human fetal membranes in vitro with Candida albicans
Abstract:
The present study was designed to examine whether C. albicans could infect and penetrate through intact human fetal membranes in vitro. Fragments of fresh fetal membranes were obtained from pregnant women undergoing a cesarean section. C. albicans was inoculated onto the surface of the maternal side of the membranes, after which the inoculated tissue were incubated at 31 degrees C. C. albicans was able to infect and penetrate through the membranes into the fetal side within 24 h of incubation.
Insights
Candida albicans can infect and penetrate intact human fetal membranes within 24 hours. This study demonstrates the potential for fungal invasion through the chorioamniotic barrier in vitro.
Area of Science:
- Obstetrics and Gynecology
- Infectious Diseases
- Microbiology
Background:
- Intact human fetal membranes serve as a protective barrier against ascending infections during pregnancy.
- Chorioamnionitis, an infection of the fetal membranes, is a significant risk factor for adverse pregnancy outcomes.
Purpose of the Study:
- To investigate the in vitro capability of Candida albicans to infect and breach intact human fetal membranes.
- To assess the potential for fungal penetration across the chorioamniotic barrier.
Main Methods:
- Human fetal membrane tissue fragments were collected from women undergoing cesarean sections.
- Candida albicans was inoculated onto the maternal side of the membrane explants.
- Tissue samples were incubated at 31°C to simulate physiological conditions.
Main Results:
- Candida albicans successfully infected the human fetal membranes.
- Fungal penetration through the membranes to the fetal side was observed within 24 hours of incubation.
Conclusions:
- Candida albicans demonstrates the ability to penetrate intact human fetal membranes in vitro.
- These findings highlight a potential mechanism for ascending infection during pregnancy.