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Related Experiment Video

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Salmonella typhimurium detection using a surface-enhanced Raman scattering-based aptasensor.

Nuo Duan1, Boya Chang1, Hui Zhang2

  • 1State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

International Journal of Food Microbiology
|November 25, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces a novel aptasensor for detecting pathogenic bacteria using surface-enhanced Raman spectroscopy (SERS). The method offers sensitive and specific detection of Salmonella typhimurium in food samples.

Keywords:
AptamerAu@Ag core/shell NPsS. typhimuriumSERS

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Area of Science:

  • Nanotechnology
  • Biosensing
  • Spectroscopy

Background:

  • Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity and specificity for biological applications.
  • Quantitative detection of pathogenic bacteria is crucial for food safety.

Purpose of the Study:

  • To develop a SERS-based aptasensor for the quantitative detection of pathogenic bacteria.
  • To functionalize a SERS substrate with aptamers for specific bacterial capture and detection.

Main Methods:

  • Fabrication of a SERS substrate with Au@Ag core/shell nanoparticles.
  • Functionalization of the substrate with aptamer 1 for target capture.
  • Utilizing X-rhodamine (ROX)-modified aptamer 2 as a recognition element and Raman reporter.
  • Formation of a sandwich-like complex for S. typhimurium detection.

Main Results:

  • The aptasensor achieved quantitative detection of Salmonella typhimurium.
  • A calibration curve was established in the range of 15 to 1.5 × 10^6 cfu/mL.
  • A limit of detection of 15 cfu/mL was achieved.
  • The method demonstrated successful application in real food samples with results consistent with plate counting.

Conclusions:

  • The developed SERS-based aptasensor enables rapid and sensitive detection of pathogenic bacteria.
  • This method shows significant potential for food safety assurance.
  • The aptasensor provides a reliable tool for monitoring bacterial contamination in food.