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Updated: Mar 29, 2026
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinoma
Meilin Qin1,2, Gang Liu3, Xisong Huo2
1Shanghai Medical College of Fudan University, Shanghai, China.
Background:
It has been shown that circular RNA (circRNA) is associated with human cancers, however, few studies have been reported in hepatocellular carcinoma (HCC).
Objective:
To estimate clinical values of a circular RNA, Hsa_circ_0001649, in HCC.
Methods:
Expression level of hsa_circ_0001649 was detected in HCC and paired adjacent liver tissues by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs). Differences in expression level of hsa_circ_0001649 were analyzed using the paired t-test. Tests were performed between clinical information and hsa_circ_0001649 expression level by analysis of variance (ANOVA) or welch t-test and a receiver operating characteristics (ROC) curve was established to estimate the value of hsa_circ_0001649 expression as a biomarker in HCC.
Results:
hsa_circ_0001649 expression was significantly downregulated in HCC tissues (p = 0.0014) based on an analysis of 89 paired samples of HCC and adjacent liver tissues and the area under the ROC curve (AUC) was 0.63. Furthermore, hsa_circ_0001649 expression was correlated with tumor size (p = 0.045) and the occurrence of tumor embolus (p = 0.017) in HCC.
Conclusions:
We first found hsa_circ_0001649 was significantly downregulated in HCC. Our findings indicate hsa_circ_0001649 might serve as a novel potential biomarker for HCC and may function in tumorigenesis and metastasis of HCC.
