[A method of biochemical identification of mutations in red adenine-dependent yeast strains]

A simple biochemical technique is proposed for quantitative estimation of expression of the ADE2 and ADE1 genes, coding for the structure of AIR-carboxylase and SAICAR-synthetase in the yeast Saccharomyces cerevisiae. The technique is based on determining the enzyme specific activities in the yeast crude extracts. The technique was applied to estimate quantitatively the expression of the ADE2 and ADE1 genes in Saccharomyces cerevisiae. The method is available for identification of mutation in the analogous genes of non-saccharomyces yeasts.