[The SERS Detection of Sudan I by Using AAO as Template to Prepare the Substrate]

The large-scale controllable, ordered two-dimensional arrays of gold nanostructure with hot-spot were prepared together with chemical molecules were modified on the surface to concentrate Sudan I within the zone of the SERS effect, which lead to analytical detection of Sudan I in high resolution. The vapor of gold was deposed on anodic aluminum oxide(AAO) template by -200 nm thickness to replicate its nanochannels, and the negative structure i. e. large-scale ordered gold nano-hemisphere array, was obtained after the removal of the template of AAO by NaOH solution. Au nano-hemisphere array was modified by 1-Dodecanethiol which can be self-assembled monolayer on the surface and concentrate Sudan I within the zone of the SERS detection, which can facilitate the measurement of Sudan I. Due to the order and regularity of Au nano-hemisphere array, the signal of Sudan I in the range of laser illumination is stable and uniform, and the quantitative analysis of Sudan I was realized. The SERS intensity of Sudan I is logistic proportional to the concentration in the range of 10(-7) to 5 x 10(-4) mol x L(-1). The corresponding correlation coefficient of the liner equation is 0.99, the recoveries of Sudan I are between 77% - 117%. The limit of detection for Sudan I is 4 x 10(-1) mol x L(-1), comparable to that of HPLC of Chinese national standard method.