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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
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Related Experiment Video

Updated: Mar 29, 2026

Setup and Execution of the Rapid Cycle Deliberate Practice Death Notification Curriculum
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Discussing Serious News.

Hilary Flint1, Mark Meyer1, Monir Hossain2

  • 11 Department of Anesthesia, Division of Pain Management, Palliative Care, Cincinnati Children's Hospital Medical Center, Cincinnati, USA.

The American Journal of Hospice & Palliative Care
|November 26, 2015
PubMed
Summary
This summary is machine-generated.

Pediatric residents showed increased confidence in communicating serious news after a workshop including bereaved parents. This novel approach enhanced realism and improved communication skills for physicians.

Keywords:
Pediatric palliative carebereaved parentscommunication skillsresident education

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Area of Science:

  • Medical Education
  • Pediatrics
  • Communication Skills Training

Background:

  • Effective communication of serious news is crucial for physicians.
  • Pediatric residents require specialized training in delivering difficult information compassionately.
  • Current communication training may lack realistic patient/family interaction.

Purpose of the Study:

  • To evaluate a novel communication skills workshop for pediatric residents.
  • To assess the impact of including bereaved parents in role-play scenarios.
  • To measure changes in residents' confidence in communicating serious news.

Main Methods:

  • A communication skills workshop was developed for pediatric residents.
  • The workshop incorporated role-playing exercises with bereaved parents.
  • Post-workshop surveys assessed residents' perceived efficacy and confidence levels.

Main Results:

  • Residents reported significant increases in confidence for discussing goals, managing emotions, and expressing empathy.
  • The inclusion of bereaved parents was perceived as highly beneficial, enhancing the realism of the training.
  • Residents believed their overall ability to communicate serious news had improved.

Conclusions:

  • A communication skills workshop involving bereaved parents effectively boosted pediatric residents' confidence.
  • The realistic simulation provided by bereaved parents significantly enhanced the training experience.
  • This intervention offers a promising model for improving physician communication in sensitive situations.