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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
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Outline of a bacterial filter-based assay to detect beta-lactamases
B B Wintermans1, C M J E Vandenbroucke-Grauls1
1Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
Journal of Microbiological Methods
|November 26, 2015
Abstract:
We describe a new phenotypic test to detect beta-lactamases. This assay is based on diffusion of beta-lactam/beta-lactamase through a bacterial filter. Beta-lactam hydrolysis on (the other side of) the filter leads to a change in antibiotic susceptibility, which can be measured by disc diffusion tests. We illustrate its ease of use to detect beta-lactamases of different classes.

