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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
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Brucella discriminates between mouse dendritic cell subsets upon in vitro infection
Alexia Papadopoulos1, Aurélie Gagnaire1, Clara Degos1
1a Centre d'Immunologie de Marseille-Luminy; Aix Marseille Université; CNRS UMR7280; INSERM U1104 ; Marseille , France.
Virulence
|November 26, 2015
Summary
Brucella bacteria infect various dendritic cell (DC) models, with GMCSF-IL15 and Flt3l DCs showing high early infection rates. Differences in IL10 secretion explain varied DC maturation responses to Brucella, aiding vaccine development.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- Brucella, a Gram-negative bacterium, causes brucellosis, a global zoonotic disease.
- Brucella infects and replicates within dendritic cells (DCs), influencing their maturation.
- In vivo studies show Brucella induces DC migration and maturation, contrasting with in vitro findings.
Purpose of the Study:
- To compare Brucella's interaction with different in vitro bone marrow-derived dendritic cell (BMDC) models.
- To elucidate how Brucella influences the maturation and cytokine secretion of distinct DC subsets.
Main Methods:
- Infection of multiple in vitro BMDC models with Brucella.
- Assessment of bacterial replication rates and DC maturation markers.
- Quantification of cytokine secretion (IL10, IL12p70, TNFα) post-infection.
Main Results:
- Brucella replicated efficiently in all tested BMDC models, with high early rates in GMCSF-IL15 DCs and Flt3l DCs.
- GMCSF-IL15 DCs and Flt3l DCs exhibited higher activation, limiting bacterial targeting to the replicative niche.
- GMCSF DCs secreted IL10, correlating with reduced IL12p70 and TNFα, and lower maturation compared to GMCSF-IL15 DCs and Flt3l DCs.
Conclusions:
- Distinct DC subsets exhibit varied responses to Brucella infection due to differences in activation and cytokine profiles.
- IL10 secretion by GMCSF DCs plays a key role in modulating DC maturation and immune response to Brucella.
- These in vitro models offer valuable tools for understanding Brucella-DC interactions and designing DC-based vaccines.
