Cell membrane and volume changes during red cell development and aging

This paper provides a summary of our understanding of cell membrane and volume changes during red cell development and aging. Cytoskeletal structures which include microtubules and microfilaments appear to play key roles in the genesis of the anucleate reticulocyte from its nucleated precursor cell, as well as in the early stages of reticulocyte development. The maturation of reticulocyte into red cell is accompanied by marked changes in cell shape and extensive remodeling of the membrane skeleton, resulting in the mature red cell acquiring a highly deformable yet remarkably stable membrane. The volume and cell density heterogeneity seen for circulating red cells also appears to be the result of the membrane changes that occur during reticulocyte maturation. Following its genesis from reticulocyte, the mature red cell undergoes further membrane and volume changes during its life span of 120 days. While it is clear that surface area loss, decrease in cell volume and cell surface modifications leading to binding of immunoglobulins accompany red cell aging, the cardinal cellular modification responsible for the removal of senescent red cells from the circulation is yet to be defined.