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Isolation, Transfection, and Culture of Primary Human Monocytes
Published on: December 16, 2019
Short Communication: HIV-1 Infection Suppresses Circulating Viral Restriction microRNAs
11 Center for Animal Experiment/ABSL-III Laboratory, State Key Laboratory of Virology, Wuhan University School of Basic Medical Sciences , Wuhan, China .
Abstract:
MicroRNAs (miRNAs) participate in host innate immunity against HIV-1 infection. We examined the impact of HIV-1 infection on viral restriction miRNAs in plasma of HIV-1-infected subjects. HIV-1-infected subjects had significantly lower plasma levels of HIV-1 restriction miRNAs (miRs-29a, -29b, -125b, -223, -198, and -382) than control subjects. Further in vitro studies showed that HIV-1 infection of macrophages suppressed production of the extracellular miRs-29b, -125b, and -223. These data demonstrate the compelling evidence that HIV-1 infection impairs host innate immunity by inhibiting antiviral miRNAs, which provide a possible mechanism for HIV-1 persistence in the host.
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